Archive

Volume 2, Number 6 / December issue 2016
Dwi Kusuma Wahyuni, Hamidah
Analysis of phytocomponents in the methanolic extract of Justicia gendarussa Burm.f.
Abstract

Justicia gendarussa Burm.f. belongs to the family Acanthaceae. In local population of West Papua, it is used as male contracetive. The objective of this study is to identify the present in the methanolic extract of Justicia gendarussa Burm.f. by GC-MS analysis. Twenty grams of the powdered plant materials are subjected to three methanol washes followed by filtration of the combined filtrate through the filter paper, and evaporate to give a final yield of 7% extract. The extract is subjected to GC-MS analysis. The GC-MS analysis of the sample is performed using an Agilent 6980N Network GC System with autosample. Data handling is done using GC-MS solution software. The identification of compounds is based on comparison of their mass spectra with those of WILEY version 8.0 Libraries. Twenty three compounds are identified. The major constituents are 2-ethylidene-1-methyl-3-phenylimidazolidine, 9,12-Octadecadienoic acid (Z,Z), and 5,6,8,9-tetramethoxy-2-methylpepero (3,4,5-jk)-9,10-dihydrophenanthracene.
Keywords: GC-MS, Justicia gendarussa Burm.f., Acanthaceae, Phytocomponents

Cite this article:
Dwi Kusuma Wahyuni, Hamidah. Analysis of phytocomponents in the methanolic extract of Justicia gendarussa Burm.f.. Acta Scientiae et Intellectus, 2(6)2016, 20-28.


REFERENCES

  1. Adnan A.Z., Z. Noer, and Zulzana, 2011. Analysis of esential oil components from leaves of Piper crocatum Ruis and Pav and Curcuma domestica Val. Majalah Farmasi dan Farmakologi, 15(1):17-22.
  2. Aparna V., K.V. Dileep, P.K. Mandal, P. Karthe, C. Sadasivan, and M. Haridas, 2012. Anti-inflammatory property of n-hexadecanoic acid: structural evidence and kinetic assessment. Chemical Biology & Drug Design, 80(3):434-439.
  3. Arun P., and J. Varsha, 2014. GC-MS analysis of bioactive components from methanol leaf extract of Toddalia asiatica (L.). Int J Pharm Sci Rev Res, 2(1):18-20.
  4. Bachheti R.K., D.P. Pandey, A. Joshi, and V. Rana, 2011. Chemical analysis of aerial parts of Justicia gendarussa. International Journal of ChemTech Research, 3(1):244-247.
  5. Chakravarty AK., PP. Dastidar, and S.C. Prakashi, 1982. Simple aromatic amines from Justicia gendarussa 13C NMR spectra of the bases and their analogues. Tetrahedron, 38: 1797-1802.
  6. Choi W.H., 2016. Evaluation of anti-tubercularactivity of linolenic acid and conjugated-linoleic acid as effective inhibitors against Mycobacterium tuberculosis. Asian Pacific Journal of Tropical Medicine, 9(2):125-129.
  7. Choi J., N. Park, S. Hwang, J.H. Sohn, I. Kwak, K.K. Cho, and I.S. Choi, 2013. The antibacterial activity of various saturated and unsaturated fatty acids against several oral pathogens. Journal of Environmental Biology, 34: 673-676.
  8. Darmstadt G.L., M. Mao-Qiang, E. Chi, SK. Saha, VA. Ziboh, RE. Black, M. Santosham, and PM. Elias, 2002. Impact of topical oils on the skin barrier: possible implications for neonatal health in developing countries. Acta Paediatrica, 91(5):546–554.
  9. Diezel W.E, E. Schulz, M. Skanks, and H. Heise, 1993. Lant oils: topical application and anti-inflammatory effects (croton oil test). Dermatologische Monatsschrift, 173-179.
  10. Hansen S.N., and W.S. Harris, 2007. New evidence for the cardiovascular benefits of long chain omega-3 fatty acids. Curr Atheroscler Rep, 9: 434-440.
  11. Harris W.S., PM. Kris-Etherton, and KA. Harris, 2008. Intakes of long-chain omega-3 fatty acid associated with reduced risk for death from coronary heart disease in healthy adults. Curr Atheroscler Rep, 10:503-509.
  12. Kavitha K., K.S. Sridevisangeetha, K. Sudjata, and S. Umamaheswari, 2014. Phytochemical and pharmacological Profile of Justicia gendarussa Burm.f.-Review. Journal of Pharmacy Research, 8(7):990-997.
  13. Killen B.U., and O.I. Corrigan, 2001. Factors influencing drug release from stearic acid based compacts. Int J Pharm, 228: 189-98.
  14. Kim E.J., J. Kwon, S.H. Park, C. Park, Y. Seo, H. Shin, H.K. Kim, K. Lee, S. Choi, D.H. Ryu, and G. Hwang, 2011. Metabolite profiling of Angelica gigas from different geographical origins using 1H NMR and UPLC-MS Analysis. J Agric Food Chem, 59: 8806–8815.
  15. Kiren Y., J. Deguchi, Y. Hirasawa, H. Morita, and BE. Prajogo, 2014. Justidrusamides A-D, new 2-Aminobenzyl alcohol derivatives from Justicia gendarussa. J Nat Med, 68: 754–758.
  16. Letawe C., M. Boone, and G.E. Pierard, 1998. Digital image analysis of the effect of topically applied linoleic acid on acne microcomedone. Clinical and Experimental Dermatology, 23(2): 56-58.
  17. Lima LARS, S. Johann, P.S. Cisalpino, L.P.S. Pimenta and M.A.D. Boaventura. 2011. In vitro antifungal activity of fatty acid methyl esters of the seeds of Annona cornifolia A.St.-Hil. (Annonaceae) against pathogenic fungus Paracoccidioides brasiliensis. Revista da Sociedade Brasileira de Medicina Tropical, 44(6):777-780.
  18. Lucie A.T., S. Dogo, L.D.P. Béranger, B.O.S. Florent, G.M. Talla, T. Anna, N. Salomon, N. Kandioura, S. Mbacké, and S. Jean-Laurent, 2013. Chemical characterization and insecticidal activity of ethyl acetate and dichloromethane extracts of Drypetes gossweileri against Sitophilus zeamais, Tribolium castaneum and Rhyzopertha dominica Journal of Life Sciences, 7(10): 1030-1040.
  19. Mahmoud F.M., S.A. Moustafa, T.H. Alamri, and A.A. Taha-Sulaiman, 2013. In vitro antifungal activity of Argemone ochroleucasweet latex against some pathogenic fungi. Afri J of Biotechnol, 12(10):1132-1137.
  20. Marliyana S.D., N. Handatani, S. Ngaisah, and N. Setyowati, 2013. Antibacterial activity of the essential oil Piper crocatum Ruiz and Pav Leaves. Alchemy Jurnal Penelitian Kimia, 2(2):33-40.
  21. Mozaffarian D. 2005. Does alpha-linolenic acid intake reduce the risk of coronary heart disease? A review of the evidence. Altern Ther Health Med, 11:24-30.
  22. Munakata K., 1983. Nematocidal natural products. In: D.L Whitehead and W.S Bowers (eds.). Natural Products for Innovative Pest Management, Oxford: Pergamon, 299-310.
  23. Natarajan V., and ASAG. Dhas, 2013. Effect of active fraction isolated from the leaf extract of Dregea volubilis [Linn.] Benth. on plasma glucose concentration and lipid profile in streptozotocin-induced diabetic rats. SpringerPlus, 2: 394.
  24. Nivas D., and DK. Gaikwad, 2014. Phytochemical screening and in-vitro antioxidant activities of Colubrina asiatica Brong. Journal of Chemical and Pharmaceutical Research, 6(9): 282-288.
  25. Ponnamma S.U., and K. Manjunath, 2012. GC-MS analysis of phytocomponents in the methanolic extract of Justicia wynaadensis (Nees) T. Anders. Int J Pharm Bio Sci, 3(3):570-576.
  26. Prajogo B.E., 2002. Aktivitas antifertilitas flavonoid daun Gendarussa vulgaris Ness. penelitian eksperimental pencegahan penetrasi spermatozoa mencit dalam proses fertilisasi in vitro. Disertation. Airlangga University. Surabaya.
  27. Prajogo B.E.W., S. Dudy, and H.S. Mulja, 2007. Analisis kadar gendarusin A pada tanaman budidaya Justicia gendarussa Burm. f. Jurnal Farmasi Indonesia, 3:176-180.
  28. Robson J., D.Q.M. Craig, and D. Deutsch 1999. An investigation into the release of cefuroxime axetil from taste-masked stearic acid microspheres. Part I: The influence of the dissolution medium on the drug release profile and the physical integrity of the microspheres. Int J Pharm, 190: 183-92.
  29. Rowe R.C., P.J. Sheskey, and M.E. Quinn, 2009. Adipic acid, in Handbook of Pharmaceutical Excipients, 6th(Ed):11–12.
  30. Satapathy A.K., G. Gunasekaran, S.C. Sahoo, K. Amit, and P.V. Rodrigues, 2009. Corrosion inhibition by Justicia gendarussa plant extract in hydrochloric acid solution. Corros Sci, 51: 2848–2856.
  31. Soerjowinoto M. and A. Poejoarinto, 1985. Laporan Perjalanan ke Jayapura Sentani (Irian Jaya). Fakultas Biologi Universitas Gajah Mada. Yogyakarta, Vol. 1.
  32. Sonala P., K. Nayanab, S. Bakulab, and S. Mamtab, 2011. Botanical identification and physicochemical investigation of leaf of Nili-Nirgundi (Justicia gendarussa). International Journal of Pharmaceutical Sciences Review and Research, 10:116-121.
  33. Sheeba G.D., and P. Viswanathan, 2014. GC-MS analysis of phytocomponents in Spermacoce Articularis L. f. leaf. Resear in Pharm, 4(4): 01-07.
  34. Syamsuhidayat SS., and J.R. Hutapea, 2000. Inventaris Tanaman Obat Indonesia Jilid I. Badan Penelitian dan Pengembangan Kesehatan. Departemen Kesehatan RI. Jakarta.
  35. Thangavel A., S. Balakrishnan, A. Arumugam, S. Duraisamy, and S. Muthusamy, 2014. Phytochemical screening, gas chromatography-mass spectrometry (GC-MS) analysis of phytochemical constituents and anti-bacterial activity of Aerva lanata (L.) leaves. African Journal of Pharmacy and Pharmacology, 8(5):126-135.
  36. Uddin M.R., S. Sinha, M.A. Hossain, M.A. Kaisar, M.K. Hossain and M.A. Rashid, 2011. Chemical and biological investigations of Justicia gendarussa (Burm. f). Dhaka Univ J Pharm Sci, 10(1):53-57.
  37. Wang YN., H.X. Wang, ZJ. Shen, L.L. Zhao, S.R. Clarke, J.H. Sun, Y.Y. Du, and G.L. Shi, 2009. Methyl palmitate, an acaricidal compound occurring in green walnut husks. Journal of Economic Entomology, 102(1):126-202.
  38. Yang Q., W. Cao, X. Zhou, Y. Xie, and S. Wang, 2014. Anti-thrombotic effects of α-linolenic acid isolated from Zanthoxylum bungeanum Maxim seeds. BMC Complementary and Alternative Medicine, 14: 348.